- For custom spotted arrays, the quantity used for analysis is most often the
log2(SampleSignalReferenceSignal)
- This ratio may or may not include background subtraction
log2(SampleSignal−SampleBackgroundReferenceSignal−ReferenceBackground)
Mikhail Dozmorov
Fall 2016
log2(SampleSignalReferenceSignal)
log2(SampleSignal−SampleBackgroundReferenceSignal−ReferenceBackground)
Condition | Fold change | Difference |
---|---|---|
B vs. A | 10 | 900 |
C vs. B | 10 | 9000 |
C vs. A | 100 | 9900 |